Simplified serum neutralization test based on enhanced green fluorescent protein-tagged classical swine fever virus.

نویسندگان

  • Yongfeng Li
  • Liang Shen
  • Yuan Sun
  • Jin Yuan
  • Junhua Huang
  • Chao Li
  • Su Li
  • Yuzi Luo
  • Hua-Ji Qiu
چکیده

The neutralization immunofluorescence test (NIFT), currently used for detecting neutralizing antibodies (NAbs) against classical swine fever virus (CSFV), is time-consuming. Here, a simplified neutralization test based on enhanced green fluorescent protein (EGFP)-tagged CSFV (EGFP-NT) was developed for direct detection of anti-CSFV NAbs without immunostaining. The relative sensitivity and specificity between EGFP-NT and NIFT or blocking enzyme-linked immunosorbent assay (ELISA) were both 100%. The NAb titers by EGFP-NT and the blocking rates by blocking ELISA showed a good correlation.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 51 8  شماره 

صفحات  -

تاریخ انتشار 2013